LN2 Alarm with LN2 Tank

DA-102, LN2 Level Alarm System

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LN2 Level Alarm

Cryomagic Freezer for IVF / Cell biology

DA-102, LN2 Level Alarm System catalog

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Cryomagic ( MD-30 SE - 30 liter )

LN2 level Alarm System

Low Liquid Nitrogen Alarm System ( LN2 Alarm )

Model : DA-102
- DA-102 can be operated by DC 9V Alkaline Battery, DC-9V Adapter and the Output port for power supply. To maximaize longevity, the unit runs for ten minutes every hour (power save mode) when the battery is used. You may use DC 9V adapter, then it is automatically changed to continuous usage (normal mode).

- DA-102 displays wide ranged informatin and secure signals such as temperature, normal, refill and alarm mute. Icon bars indicate liquid nitrogen and battery levels.

- The unit holder (clip) can be easily attached and removed to / from any side of nitrogen tank using faces tape.

Specifications
Weight : 70g
Compact Size : 92 x 61 x 23 mm
Sensor : PRT Sensor
Sensor Connector : One Touch Connector
Temperature Display : -80℃ ~ -200℃
Automatic Power Supply Change Mode :
Continuous mode when using adapter
Economic mode when using battery
Adapter : DC 9V 300mA
Battery : DC 9V Alkaline (6LR61T)
LN2 Status Display : 7 bars on LCD
Battery Status Display : 4 bars on LCD

Signals Status
Normal : LCD Display only
Refill : LCD Display and Alarm
Low LN2 Level : LCD Display and Alarm
Low Battery : LCD Display and Alarm
Open Sensor : LCD Display and Alarm
Error : LCD Display and Alarm

System configuration (Included) :
Main Body with LCD Display
Sensor with connector
Unit Holder with tape
DC 9V 300mA
DC 9V Alkaline (6LR61T)

Effects of Type IV Collagen and Laminin on theCryopreservation of Human Embryonic Stem Cells

Effects of Type IV Collagen and Laminin on theCryopreservation of Human Embryonic Stem Cells

Sun Jong Kim,a,b Jong Hyuk Park,a,b Jeoung Eun Lee,a Jin Mee Kim,a Jung Bok Lee,a,bShin Yong Moon,c Sung Il Roh,a Chul Geun Kim,b Hyun Soo YoonaaDivision of Stem Cell Biology, Medical Research Center, MizMedi Hospital; bDepartment of Life Science,College of Natural Sciences, Hanyang University; cStem Cell Research Center, Seoul,Korea

Cryopreservation of hESCs
The undifferentiated hESC clumps were harvested for routinepassaging as described above and then transferred to thefreezing medium (90% SR with 10% dimethylsulfoxide[DMSO]; frozen control group) with serial increments ofDMSO (0%, 2%, 4%, 6%, 8%, and 10%). Human type IVcollagen (Sigma, St. Louis) or human laminin (1, 2, and 5μg/ml; Sigma) were added to the freezing medium in eachexperimental group. Thirty to 40 clumps of hESCs wereloaded into a straw (IMV Technologies, L’Aigle Cedex,France) with slow freezing (at the rate of –1°C per minuteuntil –80°C, seeding at –7°C) using a cell freezer (CryoMagic, Miraebiotech, Seoul, Korea) and then stored in liquidnitrogen.

Clinical Outcomes of Frozen-thawed Embryo Transfer after

Kor. J. Fertil. Steril., Vol. 32, No. 1, 2005, 3

Clinical Outcomes of Frozen-thawed Embryo Transfer afterMicrosurgical Removal of Damaged Blastomere

Clinical Outcomes of Frozen-thawed Embryo Transfer afterMicrosurgical Removal of Damaged BlastomereWon Yun Choi, Jie Ohn Sohn, Eun A Park, Dong Ryul Lee, Woo Sik Lee, Se Yul Han,Lee Suk Park, Jung Hyun Cho, Soo Hee Kim, Kwang Yul Cha, Tae Ki YoonFertility Center of CHA General Hospital, CHA Research Institute,Pochon CHA University, Seoul, Korea

Fertilization, Embryonic Development, and Pregnancy Rate using Fresh and FrozenTesticular Sperm in Hypospermatogenesis

The Korean Andrological Society, Kor J Androl. Vol. 24, No. 2, August 2006

Fertilization, Embryonic Development, and Pregnancy Rate using Fresh and FrozenTesticular Sperm in Hypospermatogenesis

Fertilization, Embryonic Development, and Pregnancy Rate using Fresh and FrozenTesticular Sperm in HypospermatogenesisYong-Seog Park, Sun-Hee Lee, Sang Chul Han, Su Jin Choi, Jin Hyun Jun,Mi Kyoung Koong* and Ju Tae Seo†Laboratory of Reproductive Biology and Infertility, Departments of *Obstetrics and Gynecology,†Urology, Cheil General Hospital & Women's Healthcare Center,Sungkyunkwan University School of Medicine, Seoul, Korea

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Materials and Methods:

A total of 84 cycles of ICSI were performed with fresh or frozen-thawed testicular spermfrm hypospermatogenesis patients. Of these, 55 cycles (65.5%) were performed with fresh sperm, and 29 cycles(34.5%) were performed with frozen-thawed sperm. Testicular tissue was frozen with the programmed cell freezer(Cryomagic I, Miraebiotech, Seoul, Korea).
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Expression of Ganglioside GT1b in Mouse Embryos at Different

Arch Pharm Res Vol 31, No 1, 88-95, 2008DOI 10.1007/s12272-008-1125-6
Expression of Ganglioside GT1b in Mouse Embryos at Different
Developmental Stages after Cryopreservation

Bo-Hyun Kim*, Ji-Ung Jung*, Kisung Ko, Won-Sin Kim, Sun-Mi Kim, Jae-Sung Ryu, Jung-Woo Jin,Hyo-Jung Yang, Ji-Su Kim, Hyuck-Chan Kwon1, Sang-Yoon Nam2, Dong-Hoon Kwak2, Yong-Il Park3,Deog-Bon Koo4, and Young-Kug ChooDepartment of Biological Science, College of Natural Sciences, Wonkwang University, Iksan 570-749, Korea,1Obstetric and gynecology clinic, Mirae-Heemang Hospital, Seoul 135-888, Korea, 2College of Veterinary Medicineand Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Korea,3Department of Biotechnology, Catholic University, Bucheon 420-749, Korea, and 4Center for Development and Differentiation,Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea

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Freezing and thawing procedures
Slow freezing and thawing: A slow-rate freezing protocolwas employed, as described by Lassalle et al. (1985),using a programmable freezer (Cryomagic, Mirae Biotech, Seoul, Korea).
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Cryomagic - Automatic Cryopreservation Freezer

Introduction

Our most advanced controlled rate freezers are used to store a variety of critical samples of biological freezing or thawing works. The Cryomagic™ Freezers are available in 3.0 liter & 30 liter sizes. Each model includes a separate control unit, which can create and modify multiple freezing protocols with a compact thermal printer. A DPS-1800 Digital Pump can be chosen as an option, but it is standard to the 3.0 liter chamber. Unlike other analog pumps, the DPS-1800’s digital sensing system secures the samples and device itself preventing from liquid Nitrogen shortage or overheating. Comprehensive CMG 2003 software allows the user to store or retrieve the program files to a PC. At the same time the program offers password controlled access on multiple user levels, time and date stamping, program preview and verification before running and data storage for the last runs for subsequent printing.

Application Fields

◈ IVF(human embryo and sperm)
◈ Human Oocytes
◈ Animal embryo, sperm and Oocytes
◈ Aquaculture(fish sperm and embryo)
◈ Micro-organisms and viruses
◈ Bone Marrow
◈ Stem Marrow
◈ Cord Bloods
◈ Cell Lines
◈ Skin
◈ Plant and fungal cell cultures
◈ And many other applications

Features

◈ Designed for freezing of a variety of biologic samples
◈ Control platinum resistance thermometer (PRT) sensor and heater
◈ Horizontal or vertical operation (Model MD-3.0 only)
◈ 40 programs of up to 30 steps each
◈ Alpha-numeric character or graphical temperature change on LCD display of demand, sample and chamber temperatures
◈ Paper jam-less compact mini thermal printer
◈ Easy to change paper　
◈ 120 times per minute liquid Nitrogen injection for better temperature accuracy and consistency
◈ Digital sensing system for protection from the failure by liquid Nitrogen shortage
◈ Easy data storing and recording system based on Windows software

Compostions of Cryomagic

Compositions

Cryomagic Freezer consists of a chamber, control unit, digital LN2 pump & LN2 dewar and optional PC & software

Control Process

1. Microprocessor-base control unit commands digital pump preset freezing program.

2. Heated liquid nitrogen is transferred from a dewar to the chamber through the digital pump. 　

User List of Cryomagic

The User Lists of Cryomagic System

Soon-Ahn Hospital
Grace Hospital
Ilsan JE-IL Hospital
BMI Bio Science Institute
Samsung JE-IL Women's Hospital (Seoul)
Koryu University Hospital
Hyundai Women's Hospital
Bon Hospital
Sanbon JE-IL Hospital
A-Ju University
Seoul National University
Bo-Ram Hospital
Chun-Nam University Hospital
Cha-Women's Hospital
Cha-Health System (Gu-Mi)
Kun-Yang University Hospital
Macrogen
Mr.Lee Women's Hospital
Cha-Health System (Kang-Nam)
Seoul National University
Eul-Ji University
Jin Women's Hospital
Eden Women's Hospital
Shin-San Women's Hospital
Bom Women's Hospital
M & B Women's Hospital
Maria Clinic Hospital
Mizmedi Hospital (Kang-Nam)
Seoul National University (Veterinary )
Cha-Health System (Kang-Nam)
I-Code
HL Clinic
MCTT Institute
Cha Biotech
SooAm Biotech Research Foundation
Samsung JE-IL Women's Hospital (Pusan)
Mr.Hwang Women's Hospital
Mizmedi Hospital (Kang-Seo)
IL-San Baik Hospital
CL Hospital (Kwang-Ju)
MIRAE Hospital (Dae-Cheon)
Seoul National University Hospital
Cha-Health System (Bun-Dang)
Samsung JE-IL Hospital (Seoul)
Love Full Women's Hospital
Yon-Sei MoaHospital
Mizmedi Hospital ( Bio Science Institute)
Medi-I Women's Hospital
Mama-Papa Women's Hospital
Hanyang University
Samsung Medical Institute
KT Biosys
Cha-Health System (Kang-Nam)
Samsung JE-IL Hospital
Major Hospital
MiraeHeemang Women's Hospital
Cha-Health System (Bun-Dang)
SooAm Biotech Research Foundation
Dong-Kuk University Hospital ( Il-San)
Jang's Hospital
Maria Hospital (Song-Pa)