Effects of Type IV Collagen and Laminin on theCryopreservation of Human Embryonic Stem Cells

Effects of Type IV Collagen and Laminin on theCryopreservation of Human Embryonic Stem Cells

Sun Jong Kim,a,b Jong Hyuk Park,a,b Jeoung Eun Lee,a Jin Mee Kim,a Jung Bok Lee,a,bShin Yong Moon,c Sung Il Roh,a Chul Geun Kim,b Hyun Soo YoonaaDivision of Stem Cell Biology, Medical Research Center, MizMedi Hospital; bDepartment of Life Science,College of Natural Sciences, Hanyang University; cStem Cell Research Center, Seoul,Korea

Cryopreservation of hESCs
The undifferentiated hESC clumps were harvested for routinepassaging as described above and then transferred to thefreezing medium (90% SR with 10% dimethylsulfoxide[DMSO]; frozen control group) with serial increments ofDMSO (0%, 2%, 4%, 6%, 8%, and 10%). Human type IVcollagen (Sigma, St. Louis) or human laminin (1, 2, and 5μg/ml; Sigma) were added to the freezing medium in eachexperimental group. Thirty to 40 clumps of hESCs wereloaded into a straw (IMV Technologies, L’Aigle Cedex,France) with slow freezing (at the rate of –1°C per minuteuntil –80°C, seeding at –7°C) using a cell freezer (CryoMagic, Miraebiotech, Seoul, Korea) and then stored in liquidnitrogen.

Fertilization, Embryonic Development, and Pregnancy Rate using Fresh and FrozenTesticular Sperm in Hypospermatogenesis

The Korean Andrological Society, Kor J Androl. Vol. 24, No. 2, August 2006

Fertilization, Embryonic Development, and Pregnancy Rate using Fresh and FrozenTesticular Sperm in Hypospermatogenesis

Fertilization, Embryonic Development, and Pregnancy Rate using Fresh and FrozenTesticular Sperm in HypospermatogenesisYong-Seog Park, Sun-Hee Lee, Sang Chul Han, Su Jin Choi, Jin Hyun Jun,Mi Kyoung Koong* and Ju Tae Seo†Laboratory of Reproductive Biology and Infertility, Departments of *Obstetrics and Gynecology,†Urology, Cheil General Hospital & Women's Healthcare Center,Sungkyunkwan University School of Medicine, Seoul, Korea

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Materials and Methods:

A total of 84 cycles of ICSI were performed with fresh or frozen-thawed testicular spermfrm hypospermatogenesis patients. Of these, 55 cycles (65.5%) were performed with fresh sperm, and 29 cycles(34.5%) were performed with frozen-thawed sperm. Testicular tissue was frozen with the programmed cell freezer(Cryomagic I, Miraebiotech, Seoul, Korea).
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Expression of Ganglioside GT1b in Mouse Embryos at Different

Arch Pharm Res Vol 31, No 1, 88-95, 2008DOI 10.1007/s12272-008-1125-6
Expression of Ganglioside GT1b in Mouse Embryos at Different
Developmental Stages after Cryopreservation

Bo-Hyun Kim*, Ji-Ung Jung*, Kisung Ko, Won-Sin Kim, Sun-Mi Kim, Jae-Sung Ryu, Jung-Woo Jin,Hyo-Jung Yang, Ji-Su Kim, Hyuck-Chan Kwon1, Sang-Yoon Nam2, Dong-Hoon Kwak2, Yong-Il Park3,Deog-Bon Koo4, and Young-Kug ChooDepartment of Biological Science, College of Natural Sciences, Wonkwang University, Iksan 570-749, Korea,1Obstetric and gynecology clinic, Mirae-Heemang Hospital, Seoul 135-888, Korea, 2College of Veterinary Medicineand Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Korea,3Department of Biotechnology, Catholic University, Bucheon 420-749, Korea, and 4Center for Development and Differentiation,Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea

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Freezing and thawing procedures
Slow freezing and thawing: A slow-rate freezing protocolwas employed, as described by Lassalle et al. (1985),using a programmable freezer (Cryomagic, Mirae Biotech, Seoul, Korea).
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