Fertilization, Embryonic Development, and Pregnancy Rate using Fresh and FrozenTesticular Sperm in Hypospermatogenesis

The Korean Andrological Society, Kor J Androl. Vol. 24, No. 2, August 2006

Fertilization, Embryonic Development, and Pregnancy Rate using Fresh and FrozenTesticular Sperm in Hypospermatogenesis

Fertilization, Embryonic Development, and Pregnancy Rate using Fresh and FrozenTesticular Sperm in HypospermatogenesisYong-Seog Park, Sun-Hee Lee, Sang Chul Han, Su Jin Choi, Jin Hyun Jun,Mi Kyoung Koong* and Ju Tae Seo†Laboratory of Reproductive Biology and Infertility, Departments of *Obstetrics and Gynecology,†Urology, Cheil General Hospital & Women's Healthcare Center,Sungkyunkwan University School of Medicine, Seoul, Korea

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Materials and Methods:

A total of 84 cycles of ICSI were performed with fresh or frozen-thawed testicular spermfrm hypospermatogenesis patients. Of these, 55 cycles (65.5%) were performed with fresh sperm, and 29 cycles(34.5%) were performed with frozen-thawed sperm. Testicular tissue was frozen with the programmed cell freezer(Cryomagic I, Miraebiotech, Seoul, Korea).
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Expression of Ganglioside GT1b in Mouse Embryos at Different

Arch Pharm Res Vol 31, No 1, 88-95, 2008DOI 10.1007/s12272-008-1125-6
Expression of Ganglioside GT1b in Mouse Embryos at Different
Developmental Stages after Cryopreservation

Bo-Hyun Kim*, Ji-Ung Jung*, Kisung Ko, Won-Sin Kim, Sun-Mi Kim, Jae-Sung Ryu, Jung-Woo Jin,Hyo-Jung Yang, Ji-Su Kim, Hyuck-Chan Kwon1, Sang-Yoon Nam2, Dong-Hoon Kwak2, Yong-Il Park3,Deog-Bon Koo4, and Young-Kug ChooDepartment of Biological Science, College of Natural Sciences, Wonkwang University, Iksan 570-749, Korea,1Obstetric and gynecology clinic, Mirae-Heemang Hospital, Seoul 135-888, Korea, 2College of Veterinary Medicineand Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Korea,3Department of Biotechnology, Catholic University, Bucheon 420-749, Korea, and 4Center for Development and Differentiation,Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea

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Freezing and thawing procedures
Slow freezing and thawing: A slow-rate freezing protocolwas employed, as described by Lassalle et al. (1985),using a programmable freezer (Cryomagic, Mirae Biotech, Seoul, Korea).
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Cryomagic - Automatic Cryopreservation Freezer

Introduction

Our most advanced controlled rate freezers are used to store a variety of critical samples of biological freezing or thawing works. The Cryomagic™ Freezers are available in 3.0 liter & 30 liter sizes. Each model includes a separate control unit, which can create and modify multiple freezing protocols with a compact thermal printer. A DPS-1800 Digital Pump can be chosen as an option, but it is standard to the 3.0 liter chamber. Unlike other analog pumps, the DPS-1800’s digital sensing system secures the samples and device itself preventing from liquid Nitrogen shortage or overheating. Comprehensive CMG 2003 software allows the user to store or retrieve the program files to a PC. At the same time the program offers password controlled access on multiple user levels, time and date stamping, program preview and verification before running and data storage for the last runs for subsequent printing.

Application Fields

◈ IVF(human embryo and sperm)
◈ Human Oocytes
◈ Animal embryo, sperm and Oocytes
◈ Aquaculture(fish sperm and embryo)
◈ Micro-organisms and viruses
◈ Bone Marrow
◈ Stem Marrow
◈ Cord Bloods
◈ Cell Lines
◈ Skin
◈ Plant and fungal cell cultures
◈ And many other applications

Features

◈ Designed for freezing of a variety of biologic samples
◈ Control platinum resistance thermometer (PRT) sensor and heater
◈ Horizontal or vertical operation (Model MD-3.0 only)
◈ 40 programs of up to 30 steps each
◈ Alpha-numeric character or graphical temperature change on LCD display of demand, sample and chamber temperatures
◈ Paper jam-less compact mini thermal printer
◈ Easy to change paper　
◈ 120 times per minute liquid Nitrogen injection for better temperature accuracy and consistency
◈ Digital sensing system for protection from the failure by liquid Nitrogen shortage
◈ Easy data storing and recording system based on Windows software

Compostions of Cryomagic

Compositions

Cryomagic Freezer consists of a chamber, control unit, digital LN2 pump & LN2 dewar and optional PC & software

Control Process

1. Microprocessor-base control unit commands digital pump preset freezing program.

2. Heated liquid nitrogen is transferred from a dewar to the chamber through the digital pump. 　

User List of Cryomagic

The User Lists of Cryomagic System

Soon-Ahn Hospital
Grace Hospital
Ilsan JE-IL Hospital
BMI Bio Science Institute
Samsung JE-IL Women's Hospital (Seoul)
Koryu University Hospital
Hyundai Women's Hospital
Bon Hospital
Sanbon JE-IL Hospital
A-Ju University
Seoul National University
Bo-Ram Hospital
Chun-Nam University Hospital
Cha-Women's Hospital
Cha-Health System (Gu-Mi)
Kun-Yang University Hospital
Macrogen
Mr.Lee Women's Hospital
Cha-Health System (Kang-Nam)
Seoul National University
Eul-Ji University
Jin Women's Hospital
Eden Women's Hospital
Shin-San Women's Hospital
Bom Women's Hospital
M & B Women's Hospital
Maria Clinic Hospital
Mizmedi Hospital (Kang-Nam)
Seoul National University (Veterinary )
Cha-Health System (Kang-Nam)
I-Code
HL Clinic
MCTT Institute
Cha Biotech
SooAm Biotech Research Foundation
Samsung JE-IL Women's Hospital (Pusan)
Mr.Hwang Women's Hospital
Mizmedi Hospital (Kang-Seo)
IL-San Baik Hospital
CL Hospital (Kwang-Ju)
MIRAE Hospital (Dae-Cheon)
Seoul National University Hospital
Cha-Health System (Bun-Dang)
Samsung JE-IL Hospital (Seoul)
Love Full Women's Hospital
Yon-Sei MoaHospital
Mizmedi Hospital ( Bio Science Institute)
Medi-I Women's Hospital
Mama-Papa Women's Hospital
Hanyang University
Samsung Medical Institute
KT Biosys
Cha-Health System (Kang-Nam)
Samsung JE-IL Hospital
Major Hospital
MiraeHeemang Women's Hospital
Cha-Health System (Bun-Dang)
SooAm Biotech Research Foundation
Dong-Kuk University Hospital ( Il-San)
Jang's Hospital
Maria Hospital (Song-Pa)