Expression of Ganglioside GT1b in Mouse Embryos at Different

Arch Pharm Res Vol 31, No 1, 88-95, 2008DOI 10.1007/s12272-008-1125-6
Expression of Ganglioside GT1b in Mouse Embryos at Different
Developmental Stages after Cryopreservation

Bo-Hyun Kim*, Ji-Ung Jung*, Kisung Ko, Won-Sin Kim, Sun-Mi Kim, Jae-Sung Ryu, Jung-Woo Jin,Hyo-Jung Yang, Ji-Su Kim, Hyuck-Chan Kwon1, Sang-Yoon Nam2, Dong-Hoon Kwak2, Yong-Il Park3,Deog-Bon Koo4, and Young-Kug ChooDepartment of Biological Science, College of Natural Sciences, Wonkwang University, Iksan 570-749, Korea,1Obstetric and gynecology clinic, Mirae-Heemang Hospital, Seoul 135-888, Korea, 2College of Veterinary Medicineand Research Institute of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Korea,3Department of Biotechnology, Catholic University, Bucheon 420-749, Korea, and 4Center for Development and Differentiation,Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 305-806, Korea

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Freezing and thawing procedures
Slow freezing and thawing: A slow-rate freezing protocolwas employed, as described by Lassalle et al. (1985),using a programmable freezer (Cryomagic, Mirae Biotech, Seoul, Korea).
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